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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Reducing glucoamylase usage for commercial-scale ethanol production from starch using glucoamylase expressing Saccharomyces cerevisiae

Fig. 1

Construction of S. fibuligera derived glucoamylase expressing industrial S. cerevisiae strain. The map of the pYIE2-2GA-δ plasmid (a), the 5′ and 3′ were referred to as the up and down homology arm of the delta sequence, respectively. The Not I-linearized two copies of the GA expression cassette (b) and the agarose gel electrophoresis map of the verification of the GA integration, three transformants were picked randomly (c). GA1: PENO1-GA-TENO1 cassette. GA2: PADH1-GA-TPDC1. Control: pYIE2-2GA-δ plasmid. M: DNA marker. GA: Glucoamylase

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