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Table 1 Plasmids and strains used in this study

From: Reducing glucoamylase usage for commercial-scale ethanol production from starch using glucoamylase expressing Saccharomyces cerevisiae

 

Characters

Reference

Strains

  

 CCTCC M94055 (AQ)

Industrial ethanol producing S. cerevisiae strain, MAT a/α

Provided by Angel Yeast Co., Ltd

 CCTCC M94055-GA

CCTCC M94055 δ::PENO1-GA-TENO1, PADH1-GA-TPDC1

This study

 CIBTS1518

CCTCC M94055-GA derivative

This study

 CIBTS1519

CCTCC M94055-GA derivative

This study

 CIBTS1520

CCTCC M94055-GA derivative

This study

 CIBTS1521

CCTCC M94055-GA derivative

This study

 CIBTS1522

CCTCC M94055-GA derivative

This study

(CCTCC M2014657)

Plasmids

  

 pSH47-hph

Cre recombinase expression plasmid

Wang et al. (2019)

 pYIE2-2GA-δ

Glucoamylase expression plasmid

This study

 pYIE2-XKS1-PPP-δ

Backbone plasmid

Wang et al. (2019)