Table 4 Orthogonal test design and results for the production of ethanol using CIBTS1522 under high ratio of raw corn starch to water (1:2.6)
Trial | A Urea (g/100 g corn starch) | B Acid protease (U/100 g corn starch) | C Yeast inoculum (g/100 g corn starch) | D Glucoamylase (U/100 g corn starch) | Ethanol content (%, v/v) |
|---|---|---|---|---|---|
1 | 0.1 | 5 | 0.1 | 15 | 11.4 |
2 | 0.1 | 10 | 0.2 | 30 | 12.8 |
3 | 0.1 | 25 | 0.3 | 45 | 13.6 |
4 | 0.25 | 5 | 0.2 | 45 | 13.6 |
5 | 0.25 | 10 | 0.3 | 15 | 13.4 |
6 | 0.25 | 25 | 0.1 | 30 | 13.6 |
7 | 0.4 | 5 | 0.3 | 30 | 13.1 |
8 | 0.4 | 10 | 0.1 | 45 | 13.3 |
9 | 0.4 | 25 | 0.2 | 15 | 13.3 |
k1 | 12.600 | 12.700 | 12.767 | 12.700 | |
k2 | 13.533 | 13.167 | 13.233 | 13.167 | |
k3 | 13.233 | 13.500 | 13.367 | 13.500 | |
R | 0.933 | 0.800 | 0.600 | 0.800 | |
Sum of squares of deviations | 1.362 | 0.969 | 0.596 | 0.969 | |
Order | A > B = D > C |