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Table 1 Design of the experiment for investigating the glycan profile adjustment in response to supplement addition

From: Similarity assessment by multivariate statistics method based on the distance between biosimilar and originator

Run

GlcNAc (mM)a

MnCl2 (μM)a

Add in Basal or Feed Mediaa

Distanceb

SF-1

120

30

Feed

6.71

SF-2

80

30

Feed

10.62

SF-3

40

30

Feed

12.75

SF-4

120

15

Feed

8.94

SF-5

80

15

Feed

11.27

SF-6

40

15

Feed

18.93

SF-7

120

0

Feed

6.44

SF-8

80

0

Feed

6.96

SF-9

40

0

Feed

9.42

SF-10

20

0

Basal

6.06

SF-11

10

0

Basal

5.45

SF-12

5

0

Basal

6.10

SF-13

10

30

Basal

5.42

SF-14

10

15

Basal

4.25

SF-15

120

30

Feed

8.22

SF-16

80

30

Feed

9.74

SF-17

40

30

Feed

18.50

SF-18

120

15

Feed

7.78

SF-19

80

15

Feed

11.20

SF-20

40

15

Feed

20.79

SF-21

120

0

Feed

5.80

SF-22

80

0

Feed

6.40

SF-23

40

0

Feed

9.65

SF-24

20

0

Basal

5.62

SF-25

10

0

Basal

5.64

SF-26

5

0

Basal

5.48

SF-27

10

30

Basal

4.44

SF-28

10

15

Basal

4.51

SF-29

0

0

N/A

16.97

SF-30

0

0

N/A

16.95

  1. SF-1–SF-14 and SF-15–SF-28 were duplicate runs, while SF-29 and SF-30 were controls
  2. aThree factors (GlcNAc additive concentration, MnCl2 additive concentration and the addition mode) were investigated in this experiment. Three levels of GlcNAc concentration in the basal media or feed media were tested. Three MnCl2 concentrations were tested in the basal or feed media. The experiment was conducted in a shaker flask culture system.
  3. bThe column is the distance value from cluster analysis, which are the response of the experiment