Fig. 1
From: Tunnel engineering for modulating the substrate preference in cytochrome P450BsβHI
The overall structure and substrate access tunnels information of P450BsβHI. The structure model of P450BsβHI was constructed by PyMoL based on the crystal structure of P450Bsβ (PDB: 1IZO). The access tunnel identification and hydrophobicity analysis were carried out with MOLEonline (Pravda, L, 2018). a and b The structure of the P450BsβHI enzyme. Access tunnel 1 (T1) and 2 (T2) were colored in yellow and green, respectively. The B-B’ loop, B’-C loop and F-G loop were colored in orange, purple and cyan, respectively, c The hydrophobicity property of identified tunnels. Hydrophobicity index according to Hilda Cid et al from the charged residues (Glu -1.14) to nonpolar residues (Ile 1.81) (Cid et al. 1992)