Fig. 3

Evolution of the activity of P450_BsβHI variants towards the decarboxylation of lauric acid. The α-undecene products were qualitatively determined by contrasting with standard sample, and quantified by external standard method. Every bio-catalytic system contained 10 µM P450_BsβHI enzyme (or its variants), 500 µM lauric acid substrate (from a 100 mM stock solution in ethanol) and 1 mM H₂O₂ in a final volume of 1 mL of 100 mM potassium phosphate buffer (pH = 8.0). Results shown are mean ± SD of duplicated experiments