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Bioresources and Bioprocessing

Table 1 The properties of identified access tunnels in P450BsβHI

From: Tunnel engineering for modulating the substrate preference in cytochrome P450BsβHI

Tunnel

Length (Å)

Bottleneck radius (Å)

Polarity*

Tunnel 1

29

1.4

4.06

Tunnel 2

37

1.2

8.48

  1. The access tunnel analysis was carried out with MOLEonline
  2. *Polarity is calculated as an average of amino acid polarities assigned according to the method of Zimmerman et al. (1968). Polarity ranges from completely nonpolar amino acids (ALA, GLY = 0.00) through polar residues (e.g., SER = 1.67) towards charged residues (GLU = 49.90, ARG = 52.00)
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