Table 2 Regioselectivity of P450BsβHI and two best variants (BsβHI-F79A and BsβHI-F173V) towards four fatty acid substrates
From: Tunnel engineering for modulating the substrate preference in cytochrome P450BsβHI
Substrate | Enzyme | Conversion (%) | Product distribution (%) | ||
|---|---|---|---|---|---|
Alkene | α-OH-FA | β-OH-FA | |||
Lauric acid | P450BsβHI | 49.5 ± 1 | 39 | 19 | 42 |
BsβHI-F79A | 65.6 ± 2 | 55 | 24 | 21 | |
BsβHI-F173V | 70.8 ± 9 | 50 | 23 | 27 | |
Myristic acid | P450BsβHI | 28.6 ± 1 | 47 | 13 | 40 |
BsβHI-F79A | 69.2 ± 6 | 27 | 26 | 47 | |
BsβHI-F173V | 71.9 ± 4 | 34 | 9 | 57 | |
Pentadecanoic acid | P450BsβHI | 5.4 ± 1 | 60 | 16 | 24 |
BsβHI-F79A | 44.7 ± 2 | 26 | 40 | 34 | |
BsβHI-F173V | 25.3 ± 1 | 50 | 16 | 34 | |
Palmitic acid | P450BsβHI | 1.9 ± 1 | 31 | 6 | 63 |
BsβHI-F79A | 65.9 ± 3 | 14 | 16 | 71 | |
BsβHI-F173V | 26.3 ± 3 | 23 | 4 | 73 | |