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Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Carbohydrate-binding modules targeting branched polysaccharides: overcoming side-chain recalcitrance in a non-catalytic approach

Fig. 2

Model of two approaches to overcome side-chain recalcitrance dominated by debranching enzymes and CBMs, respectively. Side chains are removed by debranching enzymes prior to attacking the polysaccharide backbone in the canonical approach. With the assistance of Type III and IV CBMs, enzymes like hemicellulases that act on backbones are able to effectively and directly hydrolyze main chains in the presence of side chains, which means the recalcitrance is overcome or weakened. In both approaches, hydrolysis of main chains and side chains could be simultaneous, but it is displayed successively for better comparison between the two approaches

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