Fig. 4From: Preparation of β(1→3)/β(1→4) xylooligosaccharides from red alga dulse by two xylanases from Streptomyces thermogriseusHydrolysis of DX by StXyl10 and StXyl11. The hydrolysis products were developed by TLC, and the densities of X1 and DX were evaluated by ImageJ. The amounts of X1 and DX under hydrolysis conditions (0.5 μg/mL StXyl10 for 4 h and 2.0 μg/mL StXyl11 for 24 h) were determined by a d-xylose analysis kit and gel filtration, respectively. The amounts of X1 and DX under the other conditions were obtained by TLC densities. a DX was hydrolyzed by 0.1–2.0 μg/mL StXyl10 for 4 h at 50 °C and pH 6.5. b DX was hydrolyzed by 0.5 μg/mL StXyl10 for 1–12 h at 50 °C and pH 6.5. c DX was hydrolyzed by 0.5–8.0 μg/mL StXyl11 for 24 h at 60 °C and pH 6.5. d DX was hydrolyzed by 2.0 μg/mL StXyl11 for 1–36 h at 60 °C and pH 6.5Back to article page