A novel scale-up strategy for cultivation of BHK-21 cells based on similar hydrodynamic environments in the bioreactors

Bioresources and Bioprocessing

Table 1 Bioreactors used in this study for BHK-21 cell cultivation

Bioreactor type^a	5L-EE-0.45	5L-EE-0.5	5L-PBT-0.5	42L-EE-0.37	350L-EE-0.5	1000L-EE-0.5
Tank volume (L)	5	5	5	42	350	1000
Tank diameter (mm)	192	192	192	290	644	914
Impeller diameter (mm)	86.4	96.0	96.0	108.0	323.5	429.7
Impeller diameter/tank diameter	0.45	0.50	0.50	0.37	0.50	0.50
Culture level height (mm)	158.0	158.0	158.0	492.5	737.6	1067.6
Load volume (L)	3.50	3.50	3.50	31.0	238.0	682.0
Impeller type	EE	EE	PBT	EE	EE	EE
Number of impellers	Single	Single	Single	Single	Double	Double

^aBioreactor type is expressed as “bioreactor volume-impeller type-ratio of impeller diameter to tank diameter”