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Table 2 Ranges of agitation speeds for BHK-21 cells cultured in the different 5-L bioreactors as required by the optimal key hydrodynamic parameters

From: A novel scale-up strategy for cultivation of BHK-21 cells based on similar hydrodynamic environments in the bioreactors

Bioreactor type Key flow field parameters Agitation speed (rpm) Intersection of agitation speed (rpm)
5L-EE-0.45 \(8.5{0} \le \gamma_{{{\text{imp}}}} \le 11.0\)(s−1) 73.2 ≤ N ≤ 94.7 88.4 ≤ N ≤ 94.7
\(\varepsilon_{\tan } \ge 0.0013\)(m2 s−3) N ≥ 88.4
5L-EE-0.5 \(8.5{0} \le \gamma_{{{\text{imp}}}} \le 11.0\)(s−1) 64.0 ≤ N ≤ 82.8 75.4 ≤ N ≤ 82.8
\(\varepsilon_{\tan } \ge 0.0013\)(m2 s−3) N ≥ 75.4
5L-PBT-0.5 \(8.5{0} \le \gamma_{{{\text{imp}}}} \le 11.0\)(s−1) 89.4 ≤ N ≤ 115.7 96.3 ≤ N ≤ 115.7
\(\varepsilon_{\tan } \ge 0.0013\)(m2 s−3) N ≥ 96.3