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Table 3 Optimal hydrodynamic characteristic values of 5-L bioreactors at the optimized agitation speed ranges

From: A novel scale-up strategy for cultivation of BHK-21 cells based on similar hydrodynamic environments in the bioreactors

Bioreactor type

5L-EE-0.45

5L-EE-0.5

5L-PBT-0.5

Min

Max

Agitation speed (rpm)

88.4

94.7

75.4

82.8

96.3

115.7

γimp (s−1)

10.25

11.01

10.04

11.00

9.18

11.01

9.18

11.01

γave (s−1)

5.27

5.67

5.65

6.19

5.64

6.76

5.27

6.76

εtan (m2 s−3)

0.0013

0.0015

0.0013

0.0017

0.0013

0.0021

0.0013

0.0021

εave (m2 s−3)

0.0018

0.0021

0.0015

0.0019

0.0019

0.0029

0.0015

0.0029

EDCF (w m3 s−1)

23.58

31.20

20.42

30.01

29.48

61.32

20.42

61.32

tc (s)

2.69

2.53

1.98

1.82

2.13

1.79

1.79

2.69

KLa (h−1)

2.75

2.84

2.97

3.06

2.97

3.21

2.75

3.21

Utip (m s−1)

0.40

0.43

0.38

0.42

0.49

0.58

0.38

0.58

Uave (m s−1)

0.16

0.17

0.17

0.19

0.17

0.20

0.16

0.20