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Table 3 Optimal hydrodynamic characteristic values of 5-L bioreactors at the optimized agitation speed ranges

From: A novel scale-up strategy for cultivation of BHK-21 cells based on similar hydrodynamic environments in the bioreactors

Bioreactor type 5L-EE-0.45 5L-EE-0.5 5L-PBT-0.5 Min Max
Agitation speed (rpm) 88.4 94.7 75.4 82.8 96.3 115.7
γimp (s−1) 10.25 11.01 10.04 11.00 9.18 11.01 9.18 11.01
γave (s−1) 5.27 5.67 5.65 6.19 5.64 6.76 5.27 6.76
εtan (m2 s−3) 0.0013 0.0015 0.0013 0.0017 0.0013 0.0021 0.0013 0.0021
εave (m2 s−3) 0.0018 0.0021 0.0015 0.0019 0.0019 0.0029 0.0015 0.0029
EDCF (w m3 s−1) 23.58 31.20 20.42 30.01 29.48 61.32 20.42 61.32
tc (s) 2.69 2.53 1.98 1.82 2.13 1.79 1.79 2.69
KLa (h−1) 2.75 2.84 2.97 3.06 2.97 3.21 2.75 3.21
Utip (m s−1) 0.40 0.43 0.38 0.42 0.49 0.58 0.38 0.58
Uave (m s−1) 0.16 0.17 0.17 0.19 0.17 0.20 0.16 0.20