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Table 4 Agitation speeds calculated for the optimal hydrodynamic parameters of BHK-21 cells cultivated in 42-, 350-, and 1000-L bioreactors

From: A novel scale-up strategy for cultivation of BHK-21 cells based on similar hydrodynamic environments in the bioreactors

Hydrodynamic parameter

Optimal range of hydrodynamic parameters

Agitation speed (rpm)

42 L

350 L

1000 L

Min

Max

Min

Max

Min

Max

Min

Max

γimp (s−1)

9.18

11.01

71.7

85.9

62.7

75.2

48.1

57.6

εtan (m2 s−3)

0.0013

0.0021

68.0

85.4

56.7

68.6

41.1

51.5

γave (s−1)

5.27

6.76

80.5

103.3

55.5

71.2

50.8

65.1

εave (m2 s−3)

0.0015

0.0029

64.3

88.4

57.1

74.0

42.6

58.1

EDCF (w m−3 s−1)

20.42

61.32

65.5

91.0

59.0

82.8

51.8

69.8

tc (s)

1.79

2.69

85.6

55.8

102.6

57.4

57.1

37.6

KLa (h−1)

2.75

3.21

0.07

0.24

0.03

0.08

0.06

0.12

Utip (m s−1)

0.38

0.58

67.2

102.6

22.4

34.3

16.9

25.8

Uave (m s−1)

0.16

0.20

40.4

50.5

17.4

21.8

12.2

15.2