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Table 4 Agitation speeds calculated for the optimal hydrodynamic parameters of BHK-21 cells cultivated in 42-, 350-, and 1000-L bioreactors

From: A novel scale-up strategy for cultivation of BHK-21 cells based on similar hydrodynamic environments in the bioreactors

Hydrodynamic parameter Optimal range of hydrodynamic parameters Agitation speed (rpm)
42 L 350 L 1000 L
Min Max Min Max Min Max Min Max
γimp (s−1) 9.18 11.01 71.7 85.9 62.7 75.2 48.1 57.6
εtan (m2 s−3) 0.0013 0.0021 68.0 85.4 56.7 68.6 41.1 51.5
γave (s−1) 5.27 6.76 80.5 103.3 55.5 71.2 50.8 65.1
εave (m2 s−3) 0.0015 0.0029 64.3 88.4 57.1 74.0 42.6 58.1
EDCF (w m−3 s−1) 20.42 61.32 65.5 91.0 59.0 82.8 51.8 69.8
tc (s) 1.79 2.69 85.6 55.8 102.6 57.4 57.1 37.6
KLa (h−1) 2.75 3.21 0.07 0.24 0.03 0.08 0.06 0.12
Utip (m s−1) 0.38 0.58 67.2 102.6 22.4 34.3 16.9 25.8
Uave (m s−1) 0.16 0.20 40.4 50.5 17.4 21.8 12.2 15.2