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Table 5 The hydrodynamic parameters of large-scale bioreactors at the agitation speed calculated as the desirable hydrodynamic environment for the culture of BHK-21 cells

From: A novel scale-up strategy for cultivation of BHK-21 cells based on similar hydrodynamic environments in the bioreactors

Parameter Optimal range of hydrodynamic parameters Hydrodynamic parameters of scaled-up bioreactors
Min Max 42 L (80 rpm) 350 L (65 rpm) 1000 L (50 rpm)
γimp (s−1) 9.18 11.01 10.38 9.51 9.73
εtan (m2 s−3) 0.0013 0.0021 0.0019 0.0018 0.0021
γave (s−1) 5.27 6.76 5.30 6.17 5.29
εave (m2 s−3) 0.0015 0.0029 0.0024 0.0208 0.0022
EDCF (w m−3 s−1) 20.42 61.32 41.52 27.98 19.35
tc (s) 1.79 2.69 2.03 2.43 2.00
KLa (h−1) 2.75 3.21 6.92 10.21 11.91
Utip (m s−1) 0.38 0.58 0.46 1.10 1.15
Uave (m s−1) 0.16 0.20 0.32 0.60 0.66