A novel scale-up strategy for cultivation of BHK-21 cells based on similar hydrodynamic environments in the bioreactors

Bioresources and Bioprocessing

Table 5 The hydrodynamic parameters of large-scale bioreactors at the agitation speed calculated as the desirable hydrodynamic environment for the culture of BHK-21 cells

Parameter	Optimal range of hydrodynamic parameters		Hydrodynamic parameters of scaled-up bioreactors
Parameter	Min	Max	42 L (80 rpm)	350 L (65 rpm)	1000 L (50 rpm)
γ_imp (s⁻¹)	9.18	11.01	10.38	9.51	9.73
ε_tan (m² s⁻³)	0.0013	0.0021	0.0019	0.0018	0.0021
γ_ave (s⁻¹)	5.27	6.76	5.30	6.17	5.29
ε_ave (m² s⁻³)	0.0015	0.0029	0.0024	0.0208	0.0022
EDCF (w m⁻³ s⁻¹)	20.42	61.32	41.52	27.98	19.35
t_c (s)	1.79	2.69	2.03	2.43	2.00
K_La (h⁻¹)	2.75	3.21	6.92	10.21	11.91
U_tip (m s⁻¹)	0.38	0.58	0.46	1.10	1.15
U_ave (m s⁻¹)	0.16	0.20	0.32	0.60	0.66