Fig. 2
From: A multi-enzyme cascade for efficient production of d-p-hydroxyphenylglycine from l-tyrosine
Cascade design and in vitro reconstruction of D-HPG biosynthesis pathway. a Schematic representation of D-HPG biosynthesis from L-tyrosine through a four-enzyme cascade. b Cascade pathway protein characterization. c Analysis of the in vitro reconstructed system with LC–MS. The four-enzyme system was supplemented with 10-mM L-tyrosine, 0.5-mM NADP+, 20-mM NH4Cl, and 0.5-mM CoSO4, and each of the four purified enzymes with 10 μM