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Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: A multi-enzyme cascade for efficient production of d-p-hydroxyphenylglycine from l-tyrosine

Fig. 3

In vivo construction and optimization of multi-step cascade reactions. a Strain E. coli 01 containing double plasmids to express PmL-AAD, SambHmaS, PaMDH, and CgDAPDHBC621. b SDS-PAGE analysis of strain E. coli 01 from cell-free extracts. M, Maker; con, E. coli BL21 without overexpressing any enzymes. c Effect of substrate loading on D-HPG production by strain E. coli 01. d The specific activity of PaMDH and CgDAPDHBC621 in recombinant strains with different promoter sequences. e Effect of substrate loading on D-HPG production by strain E. coli 02. The reactions were supplemented with varying concentrations of L-tyrosine from 5 to 25 mM and 20-g/L wet cell added at 30 °C. The conversion and titer were obtained after completion of the reactions and determined by HPLC analysis. The values are averages of three experiments

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