A multi-enzyme cascade for efficient production of d-p-hydroxyphenylglycine from l-tyrosine

Bioresources and Bioprocessing

Table 1 Host strains and plasmids used in this study

Strain^a	Recombinant plasmids^b in the strain
E. coli 01	pACYC-PmL-AAD-SambHmaS, pET-PaMDH-CgDAPDH^BC621
E. coli 02	pET-_(Tac)PaMDH-CgDAPDH^BC621
E. coli 03	pET-_(T5)PaMDH-CgDAPDH^BC621
E. coli 04	pET-_(Tre)PaMDH-CgDAPDH^BC621
E. coli 05	pET-_(Trp)PaMDH-CgDAPDH^BC621
E. coli 06	pET-_(T7)PaMDH-CgDAPDH^BC621
E. coli 07	pACYC-PmL-AAD-SambHmaS, pET-_(Tac)PaMDH-CgDAPDH^{BC621/D120S/W144S/I169P}

L-AAD: l-amino acid deaminase; HmaS: 4-hydroxymandelate synthase; MDH: (S)-mandelate dehydrogenase; DAPDH: meso-diaminopimelate dehydrogenase
^aThe strains were constructed by transforming the corresponding recombinant plasmids into E. coli BL21 (DE3) express strains (New England Biolabs)
^bThe recombinant plasmids were constructed on pACYCDuet-1 or pETDuet-1 (Novagen)