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Bioresources and Bioprocessing

Table 3 Kinetic constants of purified CgDAPDHBC621 and its variants

From: A multi-enzyme cascade for efficient production of d-p-hydroxyphenylglycine from l-tyrosine

Variants

Specific activitya (U mg−1 protein)

Km (mM)

kcat (min−1)

kcat/Kmb (mM−1·min−1)

BC621

0.14 ± 0.18

20.37 ± 0.19

0.19 ± 0.37

0.009

I169P

0.32 ± 0.58

8.17 ± 0.89

1.48 ± 0.25

0.18

D120S/I169P

0.74 ± 0.21

7.83 ± 0.03

1.46 ± 0.70

0.19

W144S/I169P

0.87 ± 0.47

8.07 ± 0.68

1.35 ± 1.39

0.17

D120S/W144S/I169P

5.32 ± 0.85

2.48 ± 0.28

2.69 ± 0.30

1.08

  1. DAPDH: meso-diaminopimelate dehydrogenase
  2. a The specific activity was determined with 10-μM purified CgDAPDHBC621 or its variants and 10-mM HPGA in 1-mL Tris–HCl buffer (50-mM, pH 8.0, 20-mM NH4Cl) at 30 °C for 15 min
  3. b The kcat /Km values was determined with 10-μM purified CgDAPDHBC621 or its variants and 1–20-mM HPGA in 1-mL Tris–HCl buffer (50 mM, pH 8.0, 10-mM NH4Cl) at 30 °C for 30 min
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