Table 2 Bacterial strains and plasmids used in the study
Strains/plasmids | Application characteristics and antibiotic resistances | Sources/references |
|---|---|---|
pRJ2 | E. coli–Streptomyces shuttle plasmid used for constructing gene deletion, hygR, blaR | Zhou et al. (2018) |
pRJ5 | pIB139 derivative containing ermEp* and phiC31 attP site, hygR | This work |
pRJ65 | pRJ2 derivative used for in-frame deletion of natO, hygR, blaR | This work |
pRJ138 | pRJ2 derivative used for deletion of natJ-L, hygR, blaR | This work |
pRJ71 | PCR template used to amplify ermEp*_nat-hyg5 cassette, apramycin and thiostrepton resistances | Shen et al. (2020) |
pRJ251 | ermEp*_nat-hyg5 cassette in pRJ5 (NsiI, EcoRV), hygR, phiC31 attP site | This work |
pRJ252 | kasOp*_nat-hyg5 cassette in pRJ5 (NsiI, EcoRV), hygR, phiC31 attP site | This work |
pRJ253 | A4p_nat-hyg5 cassette in pRJ5 (NsiI, EcoRV), hygR, phiC31 attP site | This work |
pRJ255 | kasOp*_antG-natF + A4p_nat-hyg5 in pRJ5 (NsiI, EcoRV), hygR, phiC31 attP site | This work |
pRJ256 | kasOp*_antGF + A4p_nat-hyg5 in pRJ5 (NsiI, EcoRV), hygR, phiC31 attP site | This work |
RJ2 | The mutant of S. conglobatus losing conglobatin production | Shen et al. (2020) |
∆natO | In-frame deletion of natO gene from RJ2 | This work |
∆natJ-L | Deletion of natJ-L genes from RJ2 | This work |