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Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Impairing photorespiration increases photosynthetic conversion of CO2 to isoprene in engineered cyanobacteria

Fig. 1

Design and creation of an isoprene synthetic pathway in Synechocystis. The isoprene synthetic pathway is a MEP-dependent and energy consuming pathway. An exogenous isoprene synthase (IspS, from Pueraria montana) was introduced to convert DMAPP to isoprene. To optimize the isoprene-synthetic pathway, dxs, idi, ispD and ispF from E. coli were overexpressed in Synechocystis. RuBP ribulose-1,5-bisphosphate, 3PGA 3-phosphoglycerate, 2PG 2-phosphoglycolate, G3P glyceraldehyde-3-phosphate, DXP 1-deoxy-d-xylulose-5-phosphate, MEP methylerythritol phosphate, CDP-ME diphosphocytidylyl methylerythritol, CDP-MEP diphosphocytidylyl methylerythritol 2-phosphate, MEcPP methylerythritol 2,4-cyclodiphosphate, HMBPP hydroxymethylbutenyl 4-diphosphate, IPP isopentenyl pyrophosphate, DMAPP dimethylallyl pyrophosphate. Enzymes: DXS DXP synthase, DXR DXP reductoisomerase, IspD 4-diphosphocytidyl-2C-methyl-d-erythritol synthase, IspE 4-(cytidine-5′-diphospho)-2-C-methyl-d-erythritol kinase, IspF 2C-methyl-d-erythritol 2,4-cyclodiphosphate synthase, IspG 4-hydroxy-3-methylbut-2-enyl-diphosphate synthase, IspH HMBPP reductase, IDI IPP isomerase, IspS isoprene synthase

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