Fig. 3

Phenotype analysis of all strains. a Time-course of isoprene productions by the strains IspS, MEP*-IspS, IspSΔglcD1/D2 and MEP*-IspSΔglcD1/D2. b High-CO₂-requiring (HCR) phenotype analysis of the photorespiration-impaired strains ΔglcD1/D2, IspSΔglcD1/D2 and MEP*-IspSΔglcD1/D2. The strains were grown on BG11 plates with (HC) or without (LC) 50 mM NaHCO₃ under 100 μmol photons m⁻² s⁻¹ light condition. c Quantification of intracellular and extracellular glycolate in WT and all mutant strains grown on HC condition