Impairing photorespiration increases photosynthetic conversion of CO2 to isoprene in engineered cyanobacteria

Bioresources and Bioprocessing

Table 2 Measurement of chlorophyll fluorescence kinetics for photorespiration-impaired strains and WT under 588 μmol photons m⁻² s⁻¹

Strain	rETR(II)	Y(II)	rETR(I)	Y(I)	Y(ND)	Y(NA)
WT	23.63 ± 0.80	0.096 ± 0.004	56.83 ± 3.35	0.37 ± 0.01	0.59 ± 0.01	0.03 ± 0.01
ΔglcD1/D2	15.6 ± 1.35	0.063 ± 0.006	32.6 ± 4.67	0.28 ± 0.05	0.71 ± 0.07	0.01 ± 0.01
IspSΔglcD1/D2	20.03 ± 0.95	0.081 ± 0.004	47.63 ± 5.02	0.33 ± 0.01	0.65 ± 0.02	0.02 ± 0.01
MEP*-IspSΔglcD1/D2	21.63 ± 1.25	0.088 ± 0.005	56.4 ± 7.4	0.35 ± 0.01	0.63 ± 0.02	0.02 ± 0.02

Y(II), effective quantum efficiency of PSII
rETR(II), relative electron transport rate of PSII
Y(I), effective quantum efficiency of PSI
rETR(I), relative electron transport rate of PSI
Y(ND), the quantum yield of non-photochemical energy dissipation due to donor-side limitation
Y(NA), the quantum yield of non-photochemical energy dissipation due to acceptor-side limitation
Data represent the means from three independent measurements ± SD. For each experiment, three technical replicates were performed