Table 1 Transfructosylating activities of intracellular, extracellular, and total enzymes after 72 h of shake-flask cultivation with two exogenous nitrogen sources at different ratios
N1/N2a | Total (g/L) | Salt equivalent (g/L) | Cells (g/L) | Transfructosylating activity | |||||
|---|---|---|---|---|---|---|---|---|---|
N | P | NaNO3 | Na2HPO4 | Intracellular (U/mg) | Intracellular (U/mL) | Extracellular (U/mL) | Total (U/mL) | ||
0:0b | 0.92 | 0.87 | 5.6 | 4 | 8.7 ± 0.2 | 2.4 ± 0.0 | 20.9 ± 0.3 | 37.8 ± 1.5 | 58.7 ± 1.8 |
1:0 | 2.48 | 0.87 | 15 | 4 | 16.2 ± 1.2 | 3.3 ± 0.0 | 53.9 ± 1.1 | 55.9 ± 3.3 | 109.8 ± 4.4 |
3:1 | 2.48 | 0.87 | 15 | 4 | 19.8 ± 1.2 | 1.9 ± 0.0 | 37.1 ± 2.3 | 53.8 ± 5.1 | 90.9 ± 7.4 |
1:1 | 2.48 | 0.87 | 15 | 4 | 21.5 ± 0.9 | 1.7 ± 0.0 | 35.9 ± 0.9 | 46.6 ± 0.9 | 82.5 ± 4.7 |