Bioresources and Bioprocessing

Table 4 FOS yields using intracellular, extracellular and mixed enzymes from shake flasks and 1 L reactor

From: Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284

Batch	Enzyme type	FOS yield (%)
Batch	Enzyme type	1 h	3 h	6 h	12 h
Shake flask	Intracellular—cells	58.0 ± 2.0	61.2 ± 0.6	62.0 ± 0.1	54.0 ± 0.5
	Extracellular—broth	55.2 ± 1.0	58.4 ± 0.2	60. 2 ± 1.2	56.0 ± 0.9
	Mixed	56.3 ± 1.1	60.1 ± 0.5	61.5 ± 1.0	53.7 ± 0.9
1 L bioreactor	Intracellular—cells	56.3 ± 1.1	60.1 ± 0.5	61.5 ± 1.0	56.5 ± 0.8
	Extracellular—broth	59.2 ± 1.0	61.7 ± 0.5	62.7 ± 0.2	58.2 ± 0.5
	Mixed	57.7 ± 0.6	61.3 ± 0.7	61.4 ± 0.2	57.4 ± 0.6

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