Fig. 2
From: Argonaute with stepwise endonuclease activity promotes specific and multiplex nucleic acid detection
Optimization of RADAR detection. A Schematic of assay to determine single gDNA-assisted PfAgo-mediated cleavage activity. B Single gDNA-assisted PfAgo-mediated cleavage activity with gDNAs of varying concentration when the concentration of PfAgo is 0.3 µM and that of the target ssDNA is 0.8 µM. C Single gDNA-assisted PfAgo-mediated cleavage activity of PfAgo with ssDNA of varying concentration when the concentration of PfAgo is 0.3 µM and that of the gDNA is 0.8 µM. D Single gDNA-assisted PfAgo-mediated cleavage activity of PfAgo with PfAgo of varying concentration when the concentration of gDNA is 0.8 µM and that of the target ssDNA is 0.8 µM. Each bar represents the mean fluorescence signal from the fluorophore-labeled ssDNA cleavage after 40 min at 95 °C. Error bars represent the mean ± s.d., where n = 3 replicates