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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: De novo biosynthesis of C-arabinosylated flavones by utilization of indica rice C-glycosyltransferases

Fig. 3

De novo biosynthesis of schaftoside. a Reconstitution of schaftoside pathway in E. coli chases. pYH55 (Li et al. 2019) is assembled for naringenin (Nar) production and pCZ201 (Sun et al. 2020) harbors cytochrome P450 module for 2-hydroxylnaringenin (2-OHNar) production. Fermentation of sCZ113 and sCZ114 revealed similar productivity. b HPLC chromatography of the extract of sCZ113. Standard samples were also analyzed for comparison. The peak indicated in asterisk was temporarily identified as apigenin 6(8)-C-arabinoside. UV absorbance at 280 nm was monitored. (C) MS and MS/MS spectra of schaftoside (Sch) and isoschaftoside (Isosch) present in the extract of sCZ113

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