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Fig. 4 | Bioresources and Bioprocessing

Fig. 4

From: De novo biosynthesis of C-arabinosylated flavones by utilization of indica rice C-glycosyltransferases

Fig. 4

De novo biosynthesis of uncommon C-glycosides. a pYH55 (Nar module) (Li et al. 2019), pCZ201 (cytochrome P450 module) (Sun et al. 2020) and pCZ194 (arabinosylation module) were co-expressed to reconstitute apigenin di-C-arabinoside (Api-di-C-Ara) pathway in E. coli chases. b HPLC analysis of the extract of sCZ118 and HR-MS fragmentation of Api-di-C-Ara. The peak indicated in asterisk was temporarily identified as apigenin 6(8)-C-arabinoside. UV absorbance at 280 nm was monitored. c Characterization of minor C-glycosides co-eluted with Api-di-C-Ara. HR-MS and MS/MS indicated the presence of apigenin di-C-xyloside (Api-di-C-Xyl) and chrysin 6,8-C-di-arabinoside (Chr-di-C-Ara)

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