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Bioresources and Bioprocessing
Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Development and comparison of cell-free protein synthesis systems derived from typical bacterial chassis

Fig. 5

Effect of system reagent components on E. coli, B. subtilis, C. glutamate and V. natriegens CFPS systems. a Optimization process of reagent components in the system. b Mg2+. c PEP. d NTPs. e 19 AAs. f Redox environment. g PEG8000. Normalized treatment of sfGFP protein synthesis levelobtained by the system when using each reagent at a specified concentration. The mean and standard deviations are shown (N = 3). The number "1" of the normalized treatment represented the optimal fluorescence value of each factor in each CFPS system

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