Table 2 Final protein expression levels of the four CFPS systems
Bacterial species | Unoptimized system total protein (mg/L) | Optimized system total protein (mg/L) | Key factors of protein expression |
|---|---|---|---|
V. natriegens | 569.7 ± 4.1 | 725.3 ± 22.6 | Plasmid source, plasmid concentration, PEG8000, Mg2+, PEP |
C. glutamicum | 287.2 ± 25.5 | 384.6 ± 7 | Plasmid source, plasmid concentration, codon, NTPs, PEP, 19 AA |
B. subtilis | 306.1 ± 9.6 | 560.9 ± 8.7 | Plasmid source, plasmid concentration, PEG8000, PEP, Mg2+ |
E. coli | 936.1 ± 8.4 | 1435.4 ± 37 | Plasmid source, plasmid concentration, codon, 19 AA, GSSG/GSH, PEG8000 |