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Table 10 Hydrophobic interaction chromatography (HIC) in proinsulin (PI) purification

From: Downstream processing of recombinant human insulin and its analogues production from E. coli inclusion bodies

Protein Media Comments References
Denatured PI Own produced columns with end groups containing PEG 600 and phenyl Simultaneous purification and refolding of proinsulin
Buffer A: 3.0 M ammonium sulfate, 0.05 M potassium dihydrogen phosphate, pH 7.0
Buffer B: 0.05 M potassium dihydrogen phosphate, pH 7.0
Gradient elution mode
Flow rate: 1 mL/min
Bai et al. (2003)
Renatured PI NA Purification after renaturation step
Yield: 90%
Petrides et al. (1995)
  1. NA: Information not available