Table 10 Hydrophobic interaction chromatography (HIC) in proinsulin (PI) purification
Protein | Media | Comments | References |
|---|---|---|---|
Denatured PI | Own produced columns with end groups containing PEG 600 and phenyl | Simultaneous purification and refolding of proinsulin Buffer A: 3.0Â MÂ ammonium sulfate, 0.05Â M potassium dihydrogen phosphate, pH 7.0 Buffer B: 0.05Â M potassium dihydrogen phosphate, pH 7.0 Gradient elution mode Flow rate: 1Â mL/min | Bai et al. (2003) |
Renatured PI | NA | Purification after renaturation step Yield: 90% | Petrides et al. (1995) |