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Table 11 Size exclusion chromatography (SEC) in proinsulin and insulin purification

From: Downstream processing of recombinant human insulin and its analogues production from E. coli inclusion bodies

Protein

Media

Comments

References

Denatured proinsulin

TSKgel G2000SWXL

Buffer A: 8 M urea, 0.5 M NaCl, 20 mM PBS, 10 mM imidazole

Linear gradient (0–100%) elution with mobile phase B 2.0 M urea

Purity: > 79% → 93.7%

Refolding and simultaneous purification

Yuan et al. (2015)

Insulin

NA

Elution with acetic acid solution

Yield: 90%

Petrides et al. (1995)

  1. NA information not available