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Table 11 Size exclusion chromatography (SEC) in proinsulin and insulin purification

From: Downstream processing of recombinant human insulin and its analogues production from E. coli inclusion bodies

Protein Media Comments References
Denatured proinsulin TSKgel G2000SWXL Buffer A: 8 M urea, 0.5 M NaCl, 20 mM PBS, 10 mM imidazole
Linear gradient (0–100%) elution with mobile phase B 2.0 M urea
Purity: > 79% → 93.7%
Refolding and simultaneous purification
Yuan et al. (2015)
Insulin NA Elution with acetic acid solution
Yield: 90%
Petrides et al. (1995)
  1. NA information not available