Protein | Media | Comments | References |
---|---|---|---|
Denatured proinsulin | TSKgel G2000SWXL | Buffer A: 8 M urea, 0.5 M NaCl, 20 mM PBS, 10 mM imidazole Linear gradient (0–100%) elution with mobile phase B 2.0 M urea Purity: > 79% → 93.7% Refolding and simultaneous purification | Yuan et al. (2015) |
Insulin | NA | Elution with acetic acid solution Yield: 90% | Petrides et al. (1995) |