Protein | Resin ligand functional group (interactions) | Media | Comments | References |
---|---|---|---|---|
Sulfonated PI | Multimodal weak cation exchanger (ionic interaction, hydrogen bonding, and hydrophobic interaction) | Capto MMC | Binding and elution buffers contain 8Â M urea pH step elution from 5.2 to 8 Yield: 96%; purity: 82% Ability to bind sample without prior dilution | GE app. Notes 28-9966-22 AA (2012) |
Insulin | Multimodal strong anion exchanger (ionic interaction, hydrogen bonding and hydrophobic interaction) | CaptoAdhere | Elute with a buffer of pH 3.4–3.6 Two wash steps at a pH higher than the elution pH to remove the majority of the impurities from enzymatic digest | Watson et al. (2018) |