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Table 4 Oxidative sulfitolysis conditions reported in human insulin/analogue downstream processing, ranked in order of ascending duration of reaction

From: Downstream processing of recombinant human insulin and its analogues production from E. coli inclusion bodies

Duration of oxidative sulfitolysis Temp pH Sodium sulfite (A) Sodium tetrathionate (B) Molar ratio of (A): (B) Solubilization agent Suspension concentration during oxidative sulfitolysis (mg/mL) References
40 min 37 °C 8.7 0.4 M 0.15 M 2.6:1 7.5 M urea 15–20 Tikhonov et al. (2001)
1–2 h RTP 8.5–8.7 0.1 M 0.01 Ma 10:1 7 M GdnHCl NA Patrick and Lagu (1992)
2–3 h RTP 8.7 0.4 M 0.15 M 2.6:1 7.5 M urea 15–20 Tikhonov et al. (2001)
3 h RTP 8.2 0.10 M 0.01 M 10:1 7 M urea 50 Cowley and Mackin (1997)
4 h 25 °C 9.5 0.2 M 0.02 M 10:1 8 M urea 10–15 Kim et al. (2015)
4 h 25 °C 11 0.2 M 0.02 M 10:1 8 M urea 10–15 Min et al. (2011)
6 h NA 8.9 NA NA 2:1 8 M urea 3 Castellanos-Serra et al. (1996)
6 h 37 °C NA 0.8 M 0.3 M 2.6:1 8 M urea 2 Nilsson et al. (1996)
12 h 4 °C 9.5 0.2 M 0.02 M 10:1 8 M urea 10–15 Min et al. (2011)
12 h NA 9–11 3% w/w 1.5% w/w 4.9:1 6 M GdnHCl NA Petrides et al. (1995)
12 h (24 h for pretreated cells) RTP NA 0.4 M b 8 M urea and 6 M GdnHCl 10 Redwan et al. (2007)
24–48 h RTP 9.0 1.25 g/g of sample 0.55 g/g of sample 5.5:1 8 M urea NA Astolfi et al. (2004)
  1. NA information not available, RTP room temperature
  2. aPotassium tetrathionate was used
  3. b0.4 mM cystine, 1 mM copper sulfate pentahydrate, and 5 mM nickel (II) chloride hydrate were used