Table 4 Oxidative sulfitolysis conditions reported in human insulin/analogue downstream processing, ranked in order of ascending duration of reaction
Duration of oxidative sulfitolysis | Temp | pH | Sodium sulfite (A) | Sodium tetrathionate (B) | Molar ratio of (A): (B) | Solubilization agent | Suspension concentration during oxidative sulfitolysis (mg/mL) | References |
|---|---|---|---|---|---|---|---|---|
40 min | 37 °C | 8.7 | 0.4 M | 0.15 M | 2.6:1 | 7.5 M urea | 15–20 | Tikhonov et al. (2001) |
1–2 h | RTP | 8.5–8.7 | 0.1 M | 0.01 Ma | 10:1 | 7 M GdnHCl | NA | Patrick and Lagu (1992) |
2–3 h | RTP | 8.7 | 0.4 M | 0.15 M | 2.6:1 | 7.5 M urea | 15–20 | Tikhonov et al. (2001) |
3Â h | RTP | 8.2 | 0.10Â M | 0.01Â M | 10:1 | 7Â M urea | 50 | Cowley and Mackin (1997) |
4 h | 25 °C | 9.5 | 0.2 M | 0.02 M | 10:1 | 8 M urea | 10–15 | Kim et al. (2015) |
4 h | 25 °C | 11 | 0.2 M | 0.02 M | 10:1 | 8 M urea | 10–15 | Min et al. (2011) |
6Â h | NA | 8.9 | NA | NA | 2:1 | 8Â M urea | 3 | Castellanos-Serra et al. (1996) |
6 h | 37 °C | NA | 0.8 M | 0.3 M | 2.6:1 | 8 M urea | 2 | Nilsson et al. (1996) |
12 h | 4 °C | 9.5 | 0.2 M | 0.02 M | 10:1 | 8 M urea | 10–15 | Min et al. (2011) |
12 h | NA | 9–11 | 3% w/w | 1.5% w/w | 4.9:1 | 6 M GdnHCl | NA | Petrides et al. (1995) |
12 h (24 h for pretreated cells) | RTP | NA | 0.4 M | –b | – | 8 M urea and 6 M GdnHCl | 10 | Redwan et al. (2007) |
24–48 h | RTP | 9.0 | 1.25 g/g of sample | 0.55 g/g of sample | 5.5:1 | 8 M urea | NA | Astolfi et al. (2004) |