Substrates | Pretreatments | Chemical compositions | Reactor | Operation mode | VFA yields | Product compositions | Main bacterial characteristics | References |
---|---|---|---|---|---|---|---|---|
Grass clipping | Ultrasound-Ca(OH)2 | 4 and 32 g/g volatile solids (VS) glucose and xylan released | 250 mL, 35 °C, 120 rpm | Batch 12d | 578 mg/g volatile solids (VS), increase 116% | 82% acetic, 4% propionic and 4% butyric acids | Bacteria class: Clostridia and Bacteroidia | Wang et al. (2019a) |
Grass | Carbide slag | Reduced 6% lignin and 8% hemicellulose | 250 mL, 35 °C | Batch | 220 mg/g volatile solids (VS), increase 38% | 78% acetate, 4% propionate and 8% butyrate | Clostridium, Bacteroides | Tao et al. (2019) |
Napier grass | Milling | – | 250 mL flasks, 37 °C, 100 rpm, micro O2, | Batch | 107.25 mg/g volatile solids (VS) | 50–70% acetic and 20–50% propionic acids | – | Sawatdeenarunat et al. (2017) |
Japanese cedar | Vibration milling | – | 2 L, 39 °C, 30–40 rpm, pH 6.5 | Semi-continuous | 76 mg/g volatile solids (VS) | 37% acetic and 60% propionic acids | F. succinogenes (cellulolytic bacterium), P. ruminicola (synergistically with cellulolytic bacteria), S. ruminis (propionate-producing) | Agematu et al. (2017) |
Fallen leaves | Lime pretreatments | – | 250-mL flasks with 10% inoculum, 37 °C, pH 8 | Batch 5d | 1.06 g/g volatile solids (VS), increase 160% | 70% acetic and 20% butyric acids | – | Kim et al. (2013) |
Sorghum stalks | Milling and acid | Hemicellulose degraded by 60% | 135 mL, 55 °C, | Two steps | 424 mg/g volatile solids (VS), increase 84% acetate and 116% butyrate | 2.17 g/L acetic and 2.07 g/L butyric acids | – | Islam et al. (2018) |
Corn stalk | Acid pretreatment | Hemicellulose reduced by 12%, cellulose increased by 15% | 500 mL flask, 50 °C, 15 days, pH 7 | Batch | 150 mg/g volatile solids (VS) | 0.86 g/L formic, 0.59 g/L acetic, 0.62 g/L butyric and 0.64 g/L lactic acid | – | Guo et al. (2011) |
Corn stover | Hydrothermal | Lignin removal of 8% | 500 mL, 35 °C | Batch | 1.4 g/g volatile solids (VS), 3.5 times higher | 20% ethanol, 66% acetic, 8% iso-butyric acid | – | Song et al. (2019b) |
Corn stover | Wet-exploded | – | 3 L, 37 °C, 150 rpm, pH 6.5 | Batch | 1.14 g/g volatile solids (VS) | 50–70% acetate, 20–45% propionate | – | Murali et al. (2017) |
Rice straw | 1% NaOH | – | 6.5 L, pH 6, HRT 8, 9 days | Semi-continuous | 1 g/g volatile solids (VS) | 40–60% acetate, 20–33% propionate, 12% butyrate | Predominant class: Clostridia, Bacteroidia, Bacteroidetes_vadin HA17 and Deltaproteobacteria | Lu et al. (2018) |
Wheat straw | 7.4% Ca(OH)2 | Release 0.16 g Hemicellulose/g volatile solids (VS) | 600 mL, 35 °C | Batch | 223 mg/g volatile solids (VS) | 60 mg/g volatile solids (VS) acetic, 150 mg/g volatile solids (VS) butyric acid | – | Reilly et al. (2014) |
Wheat straw | – | – | 6 L, lead bed reactor, 37 °C, solid-state | Batch | 560 mg/g volatile solids (VS) | – | – | Rouches et al. (2019) |
Palm fruit bunch | Cutting | – | 40 L leach bed reactor, solid-state | Semi-continuous | 196.5 mg/gTS | – | – | Saritpongteeraka et al. (2015) |
Xylose | Acidic and thermal pretreatments | – | 500 mL, pH 6.5, 30 °C | Batch | 124 g/g volatile solids (VS), increase 187% | Acetate higher than 85% | Dominant: Clostridia, related with acidogenic and solventogenic processes | Mockaitis et al. (2020) |