Mining methods and typical structural mechanisms of terpene cyclases

Bioresources and Bioprocessing

Table 1 Kinetic parameters of terpene cyclases reported

Enzyme	Sources	K_M (μM)	k_cat (s⁻¹)	Refs.
BPPS	Salvia officinalis	1.4	–	Croteau and Karp (1979)
(+)-LS	Citrus sinensis	13.1	–	Morehouse et al. (2017)
CS	Salvia fruticosa	65.4	0.053	Kampranis et al. (2007)
EBOS	Lippia dulcis	4.8	0.04	Attia et al. (2012)
EIZS	Streptomyces coelicolor	0.7	0.045	Aaron et al. (2010)
AS	Penicillium roqueforti	0.53	0.084	Faraldos et al. (2012)
EAS	Nicotiana tabacum	2.3	0.005	Rising et al. (2000)
TS	Taxus brevifolia	3.0	0.011	Williams et al. (2000)
CPPS	Arabidopsis thaliana	3.0	0.9	Koksal et al. (2014)

BPPS bornyl diphosphate synthase, (+)-LS (+)-limonene synthase, CS 1,8-cineole synthase, EBOS (+)-epi-α-bisabolol synthase, EIZS epi-isozizaene synthase, AS aristolochene synthase, EAS 5-epi-aristolochene synthase, TS taxadiene synthase, CPPS ent-copalyl diphosphate synthase