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Fig.2 | Bioresources and Bioprocessing

Fig.2

From: Ferulic acid production by metabolically engineered Escherichia coli

Fig.2

Heterologous biosynthesis of FA in E. coli. A HPLC profiles of the fermentation products and the FA, p-coumaric acid and caffeic acid standards. B FA production by strains based on E. coli JM109(DE3) and BL21(DE3) carrying the plasmid pBR322-T7-tal-sam5-comt. The fermentation was carried out in terrific broth (TB) medium with the addition of 1 g/L L-tyrosine and 2% (v/v) glycerol at 28 °C and 250 rpm for 3 days. p-CA p-coumaric acid; FA trans-ferulic acid. C Time course of FA production from L-tyrosine by E. coli JM109(DE3) T5FA in M9Y medium supplemented with 2% (v/v) glycerol and 1 g/L L-tyrosine at 28 °C and 250 rpm for 5 days

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