Fig. 4
From: Ferulic acid production by metabolically engineered Escherichia coli
Comparison of FA biosynthesis strains co-expressed with NADPH regenerating enzymes. pSC101-T7 refers to FA biosynthesis pathway plasmid pBR322-T5-tal-sam5-comt co-expressed with plasmid pCL1920-T7 in host cell JM109(DE3); icd refers to FA biosynthesis pathway plasmid pBR322-T5-tal-sam5-comt co-expressed with plasmid pCL1920-T7-Ecicd in host cell JM109(DE3), gnd refers to FA biosynthesis pathway plasmid pBR322-T5-tal-sam5-comt co-expressed with plasmid pCL1920-T7- Ecgnd in host cell JM109(DE3), zwf refers to FA biosynthesis pathway plasmid pBR322-T5-tal-sam5-comt co-expressed with plasmid pCL1920-T7- Eczwf in host cell JM109(DE3), pntAB refers to FA biosynthesis pathway plasmid pBR322-T5-tal-sam5-comt co-expressed with plasmid pCL1920-T7- EcpntAB in host cell JM109(DE3), gapN refers to FA biosynthesis pathway plasmid pBR322-T5-tal-sam5-comt co-expressed with plasmid pCL1920-T7- CagapN in host cell JM109(DE3). The fermentation was carried out in M9Y medium supplemented with 2% (v/v) glycerol and 1 g/L L-tyrosine at 28 °C and 250 rpm for 5 days