Fig. 5
From: Ferulic acid production by metabolically engineered Escherichia coli
Effect of combination of NADPH regenerating enzyme gntAB with S-adenosyl-L-methionine synthetase metK in FA biosynthesis strain. p15a-T5 refers to FA biosynthesis pathway plasmid p15a-T5-tal-sam5-comt in host cell JM109(DE3); p15a-T5 + pSC101-T7 refers to FA biosynthesis pathway plasmid p15a-T5-tal-sam5-comt co-expressed with plasmid pCL1920-T7 in host cell JM109(DE3); p15a-T5 + pntAB refers to FA biosynthesis pathway plasmid p15a-T5-tal-sam5-comt co-expressed with plasmid pCL1920-pntAB in host cell JM109(DE3); p15a-T5 + metK refers to FA biosynthesis pathway plasmid p15a-T5-tal-sam5-comt co-expressed with plasmid pCL1920-metK in host cell JM109(DE3); p15a-T5 + pntAB + metK refers to FA biosynthesis pathway plasmid p15a-T5-tal-sam5-comt co-expressed with plasmid pCL1920-pntAB-metK in host cell JM109(DE3); The fermentation was carried out in M9Y medium supplemented with 2% (v/v) glycerol and 1 g/L L-tyrosine at 28 °C and 250 rpm for 5 days