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Table 3 Summary of Km,SAM and Ki,SAH of all the small molecule methyltransferase in BRENDA

From: Biotechnological applications of S-adenosyl-methionine-dependent methyltransferases for natural products biosynthesis and diversification

EC number Name Organism Km (µM SAM) Ki (µM SAH) Refs.
2.1.1.6 Catechol O-methyltransferase Homo sapiens 3.1 1 Rivett and Roth (1982)
2.1.1.6 Catechol O-methyltransferase Candida tropicalis 6.2 6.9 Veser (1987)
2.1.1.15 Fatty-acid O-methyltransferase Mycobacterium marinum 33.6a 0.7a Petronikolou and Nair (2015)
2.1.1.20 Glycine N-methyltransferase Oryctolagus cuniculus 200 30 Kloor et al. (2004)
2.1.1.79 Cyclopropane-fatty-acyl-phospholipid synthase Escherichia coli 90 220 Taylor and Cronan (1979)
2.1.1.95 Tocopherol C-methyltransferase Arabidopsis thaliana 5.2 4uMb Koch et al. (2003)
2.1.1.106 Tryptophan 2-C-methyltransferase Streptomyces laurentii 120 480 Frenzel et al. (1990)
2.1.1.142 Cycloartenol 24-C-methyltransferase Glycine max 32 56 Nes et al. (2003)
2.1.1.156 Glycine/sarcosine N-methyltransferase Halorhodospira halochloris 280 with sarcosine 400c Nyyssölä et al. (2001)
420 with glycin 500c
2.1.1.156 Glycine/sarcosine N-methyltransferase Aphanothece halophytica 700 with glycin 500 Waditee et al. (2003)
600 with sarcosine 300
Ectothiorhodospira halochloris 420 with glycin 500
280 with sarcosine 400
Rat 36 with glycin 80
2.1.1.157 Sarcosine/dimethylglycine N-methyltransferase Aphanothece halophytica 180 600 Waditee et al. (2003)
Ectothiorhodospira halochloris 210 with sarcosine 500
2.1.1.165 Methyl halide transferase Brassica oleracea 30 32 Attieh et al. (1995)
2.1.1.7 Nicotinate N-methyltransferase Glycine max 55 95 Upmeier et al. (1988)
2.1.1.8 Histamine N-methyltransferase Human 1.8 1.1 Francis et al. (1980)
2.1.1.9 Thiol S-methyltransferase Rat 105 144 Borchardt and Cheng (1978)
2.1.1.25 Phenol O-methyltransferase Phanerochaete chrysosporium 99 41 Coulter et al. (1993)
2.1.1.46 Isoflavone 4′-O-methyltransferase Cicer arietinum 80 30 Wengenmayer et al. (1974)
2.1.1.50 Loganate O-methyltransferase Catharanthus roseus 742.1 400 Murata et al. (2008)
2.1.1.53 Putrescine N-methyltransferase Datura stramonium L. 100 10 Walton et al. (1994)
2.1.1.53 Putrescine N-methyltransferase Hyoscyamus albus 227 110 Hibi et al. (1992)
2.1.1.67 Thiopurine S-methyltransferase Homo sapiens 2.7 0.75 Woodson and Weinshilboum (1983)
2.1.1.68 Caffeate O-methyltransferase Beta vulgaris L. 13 4 Poulton and Butt (1975)
2.1.1.68 Caffeate O-methyltransferase Glycine max 15 6.9 Poulton et al. (1976)
2.1.1.68 Caffeate O-methyltransferase Medicago sativa L. 7 2 Edwards and Dixon (1991)
2.1.1.68 Caffeate O-methyltransferase Medicago sativa L. 12 4 Edwards and Dixon (1991)
2.1.1.91 Isobutyraldoxime O-methyltransferase Pseudomonas sp. N.C.I.B. 11652 150 27 Harper and Kennedy (1985)
2.1.1.94 Tabersonine 16-O-methyltransferase Catharanthus roseus 21.7 6 Levac et al. (2008)
2.1.1.102 Demethylmacrocin O-methyltransferase Streptomyces fradiae 110 226 Kreuzman et al. (1988)
2.1.1.104 Caffeoyl-CoA O-methyltransferase Petroselinum crispum 8.2 3.5 Pakusch and Matern (1991)
2.1.1.103 Phosphoethanolamine N-methyltransferase Caenorhabditis elegans 145 9.1 Brendza et al. (2007)
2.1.1.103 Phosphoethanolamine N-methyltransferase Plasmodium falciparum 153 50c Pessi et al. (2004)
2.1.1.107 Uroporphyrinogen-III C-methyltransferase Pseudomonas denitrificans 6.3 0.32 Blanche et al. (1989)
2.1..1.136 Chlorophenol O-methyltransferase Trichoderma longibrachiatum 284 368.9 Coque et al. (2003)
2.1.1.140 (S)-Coclaurine-N-methyltransferase Tinospora cordifolia 40 62 Loeffler et al. (1995)
2.1.1.147 Corydaline synthase Corydalis cava 2.7 2.6 Rueffer et al. (1994)
2.1.1.153 Vitexin 2″-O-rhamnoside 7-O-methyltransferase Avena sativa L. 1.6 2.5 Knogge and Weissenböck (1984)
2.1.1.154 Isoliquiritigenin 2′-O-methyltransferase Medicago sativa L. 17.7 2.2 (Medicago sativa L.) Maxwell et al. (1992)
2.1.1.273 Benzoate O-methyltransferase Antirrhinum majus 28 7 Murfitt et al. (2000)
2.1.1.338 Desmethylxanthohumol 6′-O-methyltransferase Humulus lupulus 286 98 Nagel et al. (2008)
2.1.1.343 8-Amino-8-demethylriboflavin N,N-dimethyltransferase Streptomyces davaonensis 70 27 Tongsook et al. (2016)
  1. aKd
  2. bConcentration when inhibition observed
  3. cIC50 or 50% inhibition