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Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics

Fig. 2

Effects of co-culture conditions on the co-production of D-psicose and lipase. a fermentation time (2 g/L α-lactose, 10 g/L D-fructose, initial pH 6.0, 37 °C, 32 h, and inoculation ratio of recombinant B. subtilis and E. coli = 1:2); b culture temperature (2 g/L α-lactose, 10 g/L D-fructose, initial pH 6.0, 24 h, and inoculation ratio of recombinant B. subtilis and E. coli = 1:2); c initial pH (2 g/L α-lactose, 10 g/L D-fructose, 37 °C, 24 h, and inoculation ratio of recombinant B. subtilis and E. coli = 1:2); d concentration of D-fructose (2 g/L α-lactose, initial pH 6.0, 37 °C, 24 h, and inoculation ratio of recombinant B. subtilis and E. coli = 1:2); e concentration of α-lactose (10 g/L D-fructose, initial pH 6.0, 37 °C, 24 h, and inoculation ratio of recombinant B. subtilis and E. coli = 1:2); f inoculation ratio of recombinant B. subtilis and E. coli (2 g/L α-lactose, 10 g/L D-fructose, initial pH 6.0, 37 °C, and 24 h)

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