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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Secretory expression of cyclohexanone monooxygenase by methylotrophic yeast for efficient omeprazole sulfide bio-oxidation

Fig. 3

Time course of high-cell-density fermentation of strain X33-pPICZαA-CHMOAcineto-Mut (A) or BL21 (DE3)-pET-28(a) + -CHMOAcineto-Mut (C) in 5 L fermentator and the SDS-PAGE analysis of the yeast fermentation clear broth (B) or the cell-free extract of the E. coli (D) during the induction processes. For the P. pastoris fermentation, the time course was divided into the glycerol consumption , glycerol feeding and methanol induction ; and corresponding to the glycerol consumption , glycerol feeding and IPTG induction for E. coli. lane M, protein marker; the red arrow highlighted the band of CHMOAcineto-Mut

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