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Bioresources and Bioprocessing

Table 1 Kinetic profiles of recombinant CHMOAcineto-Mut expressed in E. coli and P. pastoris in pyrmetazole oxidation reactions

From: Secretory expression of cyclohexanone monooxygenase by methylotrophic yeast for efficient omeprazole sulfide bio-oxidation

Enzyme

Pyrmetazolea

NADPHb

KM (mM)

kcat (s−1)

kcat/KM (s−1 mM−1)

KM (mM)

kcat (s−1)

kcat/KM (s−1 mM−1)

CHMOAcineto-Mut-E

0.028  ±  0.004

0.35  ±  0.02

12.5

(11.2  ±  2.5)  ×  10–4

0.34  ±  0.02

304

CHMOAcineto-Mut-P

0.026  ±  0.001

1.12  ±  0.04

43.4

(7.1  ±  1.1)  ×  10–4

0.82  ±  0.02

1154

  1. aKinetic parameters were determined in 0.5 mL reaction scale which contained potassium phosphate (100 mM, pH 9.0), methanol (2%, v/v), various concentrations of pyrmetazole (2–400 μM), NADPH (200 μM) and appropriate amount of CHMOAcineto-Mut
  2. bReactions were determined by employing pyrmetazole (200 μM) and various concentrations of NADPH (5–200 μM). For detailed analytical conditions, see experimental sections
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