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Table 1 Kinetic profiles of recombinant CHMOAcineto-Mut expressed in E. coli and P. pastoris in pyrmetazole oxidation reactions

From: Secretory expression of cyclohexanone monooxygenase by methylotrophic yeast for efficient omeprazole sulfide bio-oxidation

Enzyme Pyrmetazolea NADPHb
KM (mM) kcat (s−1) kcat/KM (s−1 mM−1) KM (mM) kcat (s−1) kcat/KM (s−1 mM−1)
CHMOAcineto-Mut-E 0.028  ±  0.004 0.35  ±  0.02 12.5 (11.2  ±  2.5)  ×  10–4 0.34  ±  0.02 304
CHMOAcineto-Mut-P 0.026  ±  0.001 1.12  ±  0.04 43.4 (7.1  ±  1.1)  ×  10–4 0.82  ±  0.02 1154
  1. aKinetic parameters were determined in 0.5 mL reaction scale which contained potassium phosphate (100 mM, pH 9.0), methanol (2%, v/v), various concentrations of pyrmetazole (2–400 μM), NADPH (200 μM) and appropriate amount of CHMOAcineto-Mut
  2. bReactions were determined by employing pyrmetazole (200 μM) and various concentrations of NADPH (5–200 μM). For detailed analytical conditions, see experimental sections