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Table 3 Optimization of bio-oxidation of pyrmetazole by employing recombinant yeast secretiona
figurea

From: Secretory expression of cyclohexanone monooxygenase by methylotrophic yeast for efficient omeprazole sulfide bio-oxidation

Entry CHMOAcineto-Mut-P/BstFDH loading (U/U) Reaction scale (mL) Conversion (%)c ee (%)c,g
3 h 9 h 17 h 26 h
1 1.5/3.7 10 15 41 65 ndd > 99
2b 1.5/3.7 10 24 59 86 ndd > 99
3 4.5/11 10 32 78 > 95 ndd > 99
4b 4.5/11 10 49 98 > 99 ndd > 99
5b,e 4.5/11 10 36 67 82 ndd > 99
6e,f 450/450 600 10 30 72 97 > 99
  1. aReactions were performed in 50-mL shake flask, the reaction mixture contained potassium phosphate buffer (100 mM, pH 8.0), 100 mM sodium formate, lyophilized FDH preparation, 0.2 mM NADP+, and neutralized yeast fermentation clear broth (adjust pH to 8.0 by using 1 M K2CO3 aqueous solution), methanol (10%, v/v) and pyrmetazole (10 g L−1). The reaction mixture was incubated at 180 rpm, 25 °C
  2. bReactions were performed under oxygen atmosphere (O2-balloon)
  3. cDetermined by chiral HPLC
  4. dNot determined
  5. eThe pyrmetazole loading was increased to 20 g L−1
  6. fReaction was performed in a 1-L jacked fermenter and stirred at 150 rpm and bubbled with air (0.5 vvm)
  7. gThe ee value was determined at 9 h