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Table 2 Plasmids used in this study

From: α-Farnesene production from lipid by engineered Yarrowia lipolytica

Name

Description

Reference

pki-nat

Y. lipolytica integrative vector, hp4d promoter with XPR2 terminator, TEF promoter with CYC1 terminator, nourseothricin selection marker

This study

YLEP-nat

Y. lipolytica episomal vector, UT8 promoter with CYC1 terminator, nourseothricin selection marker

This study

pki-nat-FSERG20

pki-nat vector containing a fusion gene of codon-optimized FS and ERG20 with GGGS linker between FS and ERG20 (FSERG20) under hp4d promoter

This study

pki-nat-2FSERG20

pki-nat-FSERG20 vector containing another FSERG20 under TEF promoter

This study

pki-nat-2FSERG20-ERG12

pki-nat-2FSERG20 vector containing UT8-ERG12-CYC1 under TEF promoter

This study

YLEP-nat-ERG12

YLEP-nat vector containing ERG12

This study

YLEP-VHb

YLEP-nat vector containing VHb

This study