Fig. 2

Effects of temperature (A), pH (B), substrate molar ratios (C), and ScCR concentrations (D) on enzymatic synthesis of (R)-3A5HEF. General reaction conditions: 10 mM 3A5AF, 1 mg/mL ScCR, 0.05 mg/mL GDH cell-free extract, Mol_glucose: Mol_3A5AF  =  2:1, 0.1 mM NADH, 1 mL sodium phosphate buffer (0.1 M, pH 7) containing 5% DMSO (v/v), 35 °C, 150 r/min, 7 h; A 20–40 °C; B pH 5–9; C 1–4; D 0.2–2 mg/mL