Fig. 3

Effect of the substrate loadings on asymmetric synthesis of 3A5HEF by ScCR (A) and YueD (B). Reaction conditions: 20–100 μmol 3A5AF, 4 mg/mL enzyme, 0.05 mg/mL GDH cell-free extract, 0.1 mM NAD(P)H, 20–100 μmol CaCO₃, molar ratio of glucose to 3A5AF of 2, 1 mL sodium phosphate buffer (0.1 M, pH 7) containing 10% DMSO (v/v), 35 °C, 150 r/min. For YueD catalysis, sodium phosphate buffer (0.1 M, pH 8) was used