Bioresources and Bioprocessing

Table 1 Asymmetric synthesis of 3A5HEF catalyzed by CRs/ADHs

From: Chemoenzymatic access to enantiopure N-containing furfuryl alcohol from chitin-derived N-acetyl-D-glucosamine

Entry	Enzyme	Time (h)	Product yield (%)	Product ee (%)
1	ScCR	9	> 99	> 99 (R)
2	YueD^a	9	90	> 99 (S)
3	RalADH^a	60	12	> 99 (S)
4	AceCR	60	8	> 99 (R)
5	SynADH^a	60	6	> 99 (R)
6	HLADH	60	na^b	na

Reaction conditions: 10 mM 3A5AF, 1 mg/mL purified enzyme, 0.05 mg/mL GDH cell-free extract, 20 mM glucose, 0.1 mM NADH, 1 mL sodium phosphate buffer (0.1 M, pH 7) containing 5% (v/v) DMSO, 30 °C, 150 r/min
^aNADPH used
^bNo activity

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