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Table 1 Asymmetric synthesis of 3A5HEF catalyzed by CRs/ADHs

From: Chemoenzymatic access to enantiopure N-containing furfuryl alcohol from chitin-derived N-acetyl-D-glucosamine

Entry Enzyme Time (h) Product yield (%) Product ee (%)
1 ScCR 9 > 99 > 99 (R)
2 YueDa 9 90 > 99 (S)
3 RalADHa 60 12 > 99 (S)
4 AceCR 60 8 > 99 (R)
5 SynADHa 60 6 > 99 (R)
6 HLADH 60 nab na
  1. Reaction conditions: 10 mM 3A5AF, 1 mg/mL purified enzyme, 0.05 mg/mL GDH cell-free extract, 20 mM glucose, 0.1 mM NADH, 1 mL sodium phosphate buffer (0.1 M, pH 7) containing 5% (v/v) DMSO, 30 °C, 150 r/min
  2. aNADPH used
  3. bNo activity