Fig. 4

Schematic diagram of Rubisco carbon capture to produce d-lactate in E. coli. PRK, phosphoribulokinase, Rubisco, ribulose-1,5-bisphosphate carboxylase/ oxygenase, LDH, Lactate dehydrogenase, RuBP, ribulose 1,5-bisphosphate, 3PGA, 3-phosphoglycerate, PEP, phosphoenolpyruvate. “X” on the dotted arrows indicates that metabolic pathway was blocked. The skull and crossbones on the right side of RuBP indicates the toxicity induced by accumulation of RuBP. Orange quadrant of carboxyl carbon of the d-lactate indicates the exact position where the carbon of CO₂ is captured, and one out of four d-lactate produced, theoretically, would have captured carbon in that position