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Bioresources and Bioprocessing
Fig. 6 | Bioresources and Bioprocessing

Fig. 6

From: Discovery of a readily heterologously expressed Rubisco from the deep sea with potential for CO2 capture

Fig. 6

Characterization of Rubisco-based CO2 fixation ability in vivo. 197-2021, positive control strain with inactivated PRK and Rubisco; 7002, strain with PRK and 7002 Rubisco; RPE, strain with PRK and RPE Rubisco. Xylose, glycerol consumption a, b and d-lactate production c, d as methods described. The mean values and standard derivations of three independent repetitions were shown. P-values relative to 197–2021 indicated significance at *P < 0.05, **P < 0.01. e Plot of Rubisco carboxylation efficiency against heterologous solubility for the enzymes listed in Additional file 2: Table S3. For an organism expressing Rubisco for CO2 capture under anoxic conditions (no oxygen competition) optimality corresponds to the upper right region of the plot. Relevant engineering strategies for improving in vivo CO2 sequestration rates for different Rubisco enzymes are provided as a guide

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